1) Ion channels
Ion channels are pore-forming membrane proteins that allow ions to pass through the channel pore. Their functions include establishing a resting membrane potential, shaping action potentials and other electrical signals by gating the flow of ions across the cell membrane, controlling the flow of ions across secretory and epithelial cells, and regulating cell volume. Ion channels are present in the membranes of all cells. We are now interested in understanding function of Transient Receptor Potential (TRP) channels which have been implicated in a diverse range of cellular processes.
Autophagy is the natural, regulated mechanism of the cell that removes unnecessary or dysfunctional components. It allows the orderly degradation and recycling of cellular components. Defects in autophagy have been linked to various human diseases, including neurodegeneration and cancer, and interest in modulating autophagy as a potential treatment for these diseases has grown rapidly. Our research focuses on the regulation of autophagy by one of TRP channels in mammalian cells.
N&8208benzhydryl quinuclidine compounds are a potent and Src kinase&8208independent inhibitor of NALCN channels. Br J Pharmacol. 2020 May 20. doi: 10.1111/bph.15104.
Palmitoylation controls trafficking of the intracellular Ca2+ channel MCOLN3/TRPML3 to regulate autophagy. Autophagy. 201915(2):327-340. doi:10.1080/15548627.2018.1518671
Dual action of the Gαq-PLCβ-PI(4,5)P2 pathway on TRPC1/4 and TRPC1/5 heterotetramers. Sci Rep. 2018 Aug 148(1):12117.
TLR3-/4-Priming Differentially Promotes Ca(2+) Signaling and Cytokine Expression and Ca(2+)-Dependently Augments Cytokine Release in hMSCs. Sci Rep. 2016 Mar 166:23103.
The Ca2+ channel TRPML3 specifically interacts with the mammalian ATG8 homologue GATE16 to regulate autophagy. Biochem Biophys Res Commun. 2014443(1):56-61. doi:10.1016/j.bbrc.2013.11.044
Isoform- and receptor-specific channel property of canonical transient receptor potential(TRPC)1/4 channels. Pflugers Arch. 2014 Mar:466(3):491-504.
Activation of TRPC4β by Gαi subunit increases Ca2+ ivity and controls neurite morphogenesis in cultured hippocampal neuron. Cell Calcium. 2013 Oct54(4):307-19.
ive Gαi subunits as novel direct activators of TRPC4 and TRPC5 channels. J Biol Chem. 2012 May 18287(21):17029-39.
Properties of the TRPML3 pore and its stable expansion by the varitint-waddler causing mutation. J Biol Chem. 2010 May 28285(22):16513-20.